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Methylene Blue CAS: 7220-79-3 | Molecular formula: C16H18ClN3S | Molecular weight: 319.85 g/mol.

Información del Producto

Methylene Blue – CAS 7220-79-3

Methylene Blue (CAS 7220-79-3), also known as Methylthioninium chloride trihydrat, is supplied by Rexar as a research-grade chemical reference material for analytical chemistry, compound identification and laboratory comparison workflows. This compound is provided for controlled research environments where verified chemical identity, reproducible analytical behaviour and consistent documentation standards are required.

Methylene Blue is available directly through the Rexar webshop and supplied in sealed laboratory packaging for distribution within the European Union.

Rexar Technical Compound Datasheet (PDF)

Safety Data Sheet (SDS)

Public reference

Methylene Blue on PubChem

Comprehensive structural overview

Methylene Blue is a synthetic phenothiazine dye belonging to a class of heterocyclic aromatic compounds characterised by a phenothiazinium core structure. The molecular framework consists of a tricyclic phenothiazine ring system incorporating both nitrogen and sulfur atoms within the aromatic scaffold.

The molecule contains dimethylamino substituents attached to the aromatic rings and exists as a positively charged phenothiazinium ion paired with a chloride counterion. This structure contributes to defined electronic properties, strong coloration and characteristic spectroscopic behaviour.

Phenothiazine class context

Phenothiazine derivatives are characterised by a heterocyclic tricyclic ring system containing both sulfur and nitrogen atoms. Structural variations arise through substitution on the aromatic rings and modification of side groups attached to the heterocyclic core.

These aromatic heterocycles exhibit conjugated π-electron systems that influence optical absorption and electrochemical behaviour, making them suitable reference compounds in analytical workflows.

Functional group analysis

  • Aromatic phenothiazine ring system
  • Tertiary amine groups
  • Heterocyclic sulfur atom
  • Positively charged phenothiazinium center

Spectroscopic characteristics

NMR spectroscopy: Proton NMR spectra typically show aromatic proton resonances associated with the phenothiazine ring system along with signals corresponding to the dimethylamino substituents.

Infrared spectroscopy: Infrared spectra display characteristic aromatic ring vibrations and signals associated with tertiary amine functionality.

UV-Vis spectroscopy: The extended conjugated aromatic system produces strong absorption in the visible spectrum, which accounts for the compound’s intense blue coloration in solution.

Mass spectrometric analysis

Mass spectrometry confirms a molecular weight of 319.85 g/mol for the chloride salt form of the compound.

Solid-state characteristics

Methylene Blue typically appears as a dark green crystalline powder that forms an intense blue solution when dissolved in water.

Registry information and structural identifiers

  • Chemical name: Methylthioninium chloride
  • Synonym: Methylene Blue
  • CAS number: 7220-79-3
  • Molecular formula: C16H18ClN3S
  • Molecular weight: 319.85 g/mol
  • Physical form: Dark green crystalline powder

 

Disclaimer

This product is intended for laboratory research use only. It is not intended for human or animal consumption, nor for medical, diagnostic or therapeutic applications.

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Especificaciones

Intended use: Laboratory research and analytical reference purposes only
Application area: Analytical chemistry, reference comparison and method development
End user: Professional users in controlled research environments
Regulatory classification: Chemical reference material
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